Create virtualhost manually

I created a virtual host file:
<VirtualHost *:80>
ServerName indextest.com
DocumentRoot /var/www/index/
ServerAlias www.indextest.com
<Directory /var/www/index>
Order deny,allow
Allow from all
Options Indexes FollowSymLinks

    ErrorLog ${APACHE_LOG_DIR}/error.log

    # Possible values include: debug, info, notice, warn, error, crit,
    # alert, emerg.
    LogLevel debug

    CustomLog ${APACHE_LOG_DIR}/access.log combined

But accessing to www.indextest.com:
#########
Forbidden

You don’t have permission to access / on this server.
#################

ls -la /var/www/index:
##########
total 8
drwxr-xr-x 2 www-data www-data 4096 Jun 5 15:52 .
drwxr-xr-x 12 root root 4096 Jun 5 15:34 …
-rw-r–r-- 1 www-data www-data 177 Jun 5 16:36 index.html
-rw-r–r-- 1 www-data www-data 0 Jun 5 15:52 test1.html
-rw-r–r-- 1 www-data www-data 0 Jun 5 15:52 test2.html
-rw-r–r-- 1 www-data www-data 0 Jun 5 15:52 test3.html
#############

I tried to copy the file index.html in /home/domain/public_html/ created with virtualmin and it works.

Do you know which directive block the virtual host indetest.com?

Thank you

No idea? Very strange, I will create another virtual machine with default conf. If it works I will diff the conf files and I let you know.
A question: Is there a way to get verbose infos about why Apache return “Forbidden”? I tried to set all logs to debug level but I got unuseful infos about the module wsgi.

same configuration /etc/apache2 in new virtual machine works in the old one continue to not working.
Please HELP!!!